首页|期刊导航|中国海洋湖沼学报(英文版)|MOLECULAR CLONING AND SEQUENCING OF A cDNA ENCODING PARTIAL PUTATIVE MOLT-INHIBITING HORMONE FROM PENAEUS CHINENSIS
中国海洋湖沼学报(英文版)2002，Vol.20Issue(3)：232-236,5.
MOLECULAR CLONING AND SEQUENCING OF A cDNA ENCODING PARTIAL PUTATIVE MOLT-INHIBITING HORMONE FROM PENAEUS CHINENSIS
MOLECULAR CLONING AND SEQUENCING OF A cDNA ENCODING PARTIAL PUTATIVE MOLT-INHIBITING HORMONE FROM PENAEUS CHINENSIS
摘要
Abstract
Total RNA was extracted from eyestalks of shrimp Penaeus chinensis. Eyestalk cDNA was obtained from total RNA by reverse transcription. Reverse transcriptase-polymer ase chain reaction (RT-PCR) was initiated using eyestalk cDNA and degenerate primers designed from the amino acid sequence of molt-inhibiting hormone from shrimp Penaeus japonicus. A s pecific cDNA was obtained and cloned into a T vector for sequencing. The cDNA consisted of 201 ba se pairs and encoding for a peptide of 67 amino acid residues. The peptide of P. chinensis had the highest identity with molt-inhibiting hormones of P. japonicus. The cDNA could be a partial gene of molt-inhibiting hormones from P. chinensis. This paper reports for the first time cDNA encoding for neuropeptide of P. chinensis.关键词
Penaeus chinensis/molt-inhibiting hormone/complementary DNA/molecular cloning/sequencingKey words
Penaeus chinensis/molt-inhibiting hormone/complementary DNA/molecular cloning/sequencing分类
生物科学引用本文复制引用
王在照,相建海..MOLECULAR CLONING AND SEQUENCING OF A cDNA ENCODING PARTIAL PUTATIVE MOLT-INHIBITING HORMONE FROM PENAEUS CHINENSIS[J].中国海洋湖沼学报(英文版),2002,20(3):232-236,5.基金项目
The work was supported by the Chinese National Key Fun damental Research Program (No. G1999012007) and the National Foundation of Science (40106018). （No. G1999012007）
