南京大学学报（自然科学版）2004，Vol.40Issue(2)：232-244,13.
以多巴胺为配体示差脉冲伏安法间接测定生物体液中的铝
Differential Pulse Voltammetric Indirect Measurement of Aluminum in Biological Fluids Using Dopamine as Sequestering Agent
摘要
Abstract
Neurotransmitter dopamine (3, 4-dihydroxyphenylethylamine, DA) has been adopted as an electroactive sequestering agent of aluminum (Al) for indirect determination of Al in biological fluids. It was observed that the decrease of the differential pulse voltammetric (DPV) anodic peak current of DA was linear with the increase of Al in the pH range from 6.0 to 7.6. Under optimum experimental conditions (pH 7.0, 2.4 × 10-4 mol/L DA, and 0.08 mol/L NH4Ac buffer solution),the detection limit of Al in the linear range from 4.0 × 10-7 to 42 × 10-7 mol/L is 1.7 × 10-7 mol/L and the relative standard deviation for 4.0 × 10-6 mol/L AlⅢ is 3.4% ( n = 8). Many foreign species possibly existing in biological fluids have been tested for interference. Recoveries between 95 %～108 % have been achieved in the application of the method to measurements of Al in biological samples such as synthetic renal dialysate, Ringer's solution, human blood, cerebrospinal fluid of patients, and urine of diabetic patients. The methodological principle that Al is complexed with DA on the electroactive site resulting in the depression of electroactive DA has been verified from the electrochemical evidences and the responses of UV-vis, Raman, and 13C NMR of DA both in the presence and absence of Al. This principle is applicable to both the chelants with the same electroactive functional groups as DA and similar cases such as monitoring electrochemical inactive species by electroactive ligands through complexation reactions.关键词
铝/多巴胺/示差脉冲伏安法Key words
aluminum, 3, 4-dihydroxyphenylethylarnine (dopamine),differential pulse voltammetry分类
社会科学引用本文复制引用
章福平,刘剑,毕树平,顾晓东,马朋举,杨小弟,王先龙,杨立,郁清..以多巴胺为配体示差脉冲伏安法间接测定生物体液中的铝[J].南京大学学报（自然科学版）,2004,40(2):232-244,13.基金项目
National Natural Science Foundation of China (20075010), the Research Fundings of Electroanalytical Chemistry Laboratory of the Changchun Applied Chemistry Institute, the Research Fundings of the Chinese Academy of Sciences, the State Key Laboratory of Co （20075010）
