中国现代医学杂志2001，Vol.11Issue(3)：23-25,3.
"一肾一夹"高血压大鼠心肌钠泵α亚单位的基因表达
ISOFORM-SPECIFIC REGULATION OF SODIUM PUMPα-SUBUNIT GENE EXPRESSION IN MYOCARDIUM OF 1-KIDNFY-1-CLIP HYPERTENSIVE RAT
摘要
Abstract
Objective:To investigate the regulation of sodium pump α-subunit gene expression in myocardium of 1-kidney-1-clip (lklc) hypertensive rat. Methods: lklc hypertensive rats were prepared by partially ligating the left renal artery and removingthe right kidney. 4 weeks later,all the rats were killed and sodium pump α1 -, α2 -, and α3 -subunit in myocardium were detectedwith reverse transcription polymerase chain reaction (RI-PCR) method and immunohistochemical assay at mRNA and protein levels,respectively. Results:There were great changes in the expression of sodium pump α-subunit gene in myocardium ol lklc hypertensive rat. α2-subunit decreased and α3 -subunit increased at mRNA level, while only the α2 -subunit increased at protein level.Conclusions:There were great changes in the expression of sodium pump α-subunit gene in myocardium of lklc hypertensive rat,inthe expression of sodium pump α-subunit gene in myocardium of lklc hypertensive rat, which might be related to the development ofhypertension in this hypertensive model.关键词
高血压/一肾一夹/大鼠/心肌/钠泵分类
医药卫生引用本文复制引用
原卫清,王颢,吕卓人.."一肾一夹"高血压大鼠心肌钠泵α亚单位的基因表达[J].中国现代医学杂志,2001,11(3):23-25,3.
