中国现代医学杂志2004，Vol.14Issue(20)：23-26,29,5.
缺血再灌注大鼠肠上皮细胞线粒体DNA ATPase 6,8亚基基因的改变
Study on changes of mitochondrial DNA ATPase 6,8 genes of small intestine epithelial cells in ischemia-reperfusion rats
李伟文 1陆松敏 1刘建仓 1武凡 1柏干荣 1李萍1
作者信息
- 1. 第三军医大学附属大坪医院野战外科研究所第二研究室,重庆,400042
- 折叠
摘要
Abstract
Objective: To observe the changes of mitochondrial DNA ATPase 6, 8 genes of small intestine epithelial cells in ischemia-reperfusion rats. Methods: 6 rats were randomly divided into the control and ischemia-reperfusion group. Their mtDNA were distilled and the sequences were examined after polymerase chain reaction conducted by using special primer in order to detect mutational sites. Results: The polymorphism existed at some sites of ATPase 6, 8 genes of Wistar rats in China. There was a A7 797 deletion and several sites mutation of ATPase 6,8 genes in ischemia-reperfusion group, namely, A7 779T, T8 515G, T8 520G, C8 535G. Conclusion: The damage of ATPase 6, 8 genes took place after ischemia-reperfusion and this may result in the changes of their coding amino acids.关键词
早期复苏/线粒体DNA/ATPase 6基因/ATPase 8基因/多态性/突变Key words
ischemia-reperfusion/mtDNA/ATPase 6 gene/ATPase 8 gene/Polymorphism/mutation分类
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李伟文,陆松敏,刘建仓,武凡,柏干荣,李萍..缺血再灌注大鼠肠上皮细胞线粒体DNA ATPase 6,8亚基基因的改变[J].中国现代医学杂志,2004,14(20):23-26,29,5.
