农业生物技术学报2001，Vol.9Issue(1)：23-27,5.
大麦黄矮病毒单双价外壳蛋白基因植物表达载体构建及小麦遗传转化
Construction of Plant Expression Vectors and Wheat Genetic Transformation of Barley Yellow Dwarf Virus Single and Double Coat Protein Genes
贾力 1吴茂森 1张文蔚 1肖红 1成卓敏1
作者信息
摘要
Abstract
PAV Chinese isolate of BYDV isolated and purified from our national wheat.The coat protein(CP) gene of this isolate was identified by reverse transcription polymerase chain reaction(RT-PCR),clone and s
equence.The cloned fragment contains a single open reading frame of 00 bp which encode 199 amino acid.PAV CP was cloned into expression ector pEmu-mcs-N to construct the plant expression vector pPPI10.In the term of the two kinds of cloned CP genes of PAV and GPV,we onstructed plant expression vector pPPI14 which contain CP genes of PAV and GPV,then transformed the two kinds of plasmids into wheat cultivar Beijing 837 by pollen tube pathway.Transgenic plants were confirmed by Kanamycin selection,dot blot,PCR and Southern blot assay,the transformation frequence is 2.5% and 0.36%,respectively.Most of transgenic plants showed reduction in virus accumulation observed upon virus infection.Compared with the control delay in symptom development BYDV resistance inoculation in the greenhouse and fields,so we can conclude that transgenic plants gained certain resistance.关键词
大麦黄矮病毒/PAV中国分离物/外壳蛋白基因/花粉管通道法/转基因小麦分类
农业科技引用本文复制引用
贾力,吴茂森,张文蔚,肖红,成卓敏..大麦黄矮病毒单双价外壳蛋白基因植物表达载体构建及小麦遗传转化[J].农业生物技术学报,2001,9(1):23-27,5.
