首页|期刊导航|中华医学杂志（英文版）|Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues
中华医学杂志（英文版）2003，Vol.116Issue(2)：235-238,4.
Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues
Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues
摘要
Abstract
Objective To investigate the expression sequence and distribution characteristics of the protooncogenes c-fos, c-myc and endogenous basic fibroblast growth factor (bFGF ) genes in burned tissues, and to explore the possible effects of changes in the se genes' functions on wound healing. Methods Partial-thickness burns of 30% TBSA were established on backs of Wistar rats. Insitu hybridization and histological methods were used to detect expression of c-fos, c-myc and bFGF genes in normal and burned tissue at 3 h, 6 h, 1 d, 3 d , 7 d and 14 d postburn. Results Although expression of c-fos and c-myc genes and bFGF gene could be found in normal skin, the expression of all three were markedly induced by burn wounds and the expression models in sequence and distribution were quite different. Expre ssion of c-fos gene increased and peaked at 6 h. Signals were mainly localiz ed in both nuclei of dermal fibroblasts and monocytes. The expression of bFGF gene increased at 6 h and peaked at 1 d postburn, and was distributed in the cyt oplasm of fibroblasts. C-myc gene peaked 3 d postburn and was also distributed in the cytoplasm of fibroblasts. Conclusions These results indicated that thermal injury could induce the expression of c-fos, c-myc and bFGF at gene level, showing phasic control and regional distributi on. The phasic expression of these genes suggests that there is an interaction between protooncogenes and bFGF, which may play an important role in wound heali ng. The different expressions of c-fos and c-myc play an inducing role in reg ulating bFGF, and in turn affect wound healing.关键词
protooncogenes/fibroblast growth factor/burn/network regulationKey words
protooncogenes/fibroblast growth factor/burn/network regulation分类
医药卫生引用本文复制引用
付小兵,顾小曼,孙同柱,杨银辉,孙晓庆,盛志勇..Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues[J].中华医学杂志（英文版）,2003,116(2):235-238,4.基金项目
This work was supported in part by the National Outstanding Young Researcher (No. 39525024), National Nature and Science Foundation (No. 301909766) and Nat ional "973" Program (No. G1999054204). （No. 39525024）
