中华医学杂志（英文版）2004，Vol.117Issue(2)：235-240,6.
Effect of human osteopontin on proliferation, transmigration and expression of MMP-2 and MMP-9 in osteosarcoma cells
Effect of human osteopontin on proliferation, transmigration and expression of MMP-2 and MMP-9 in osteosarcoma cells
刘思金 1胡国法 1刘亚军 1刘思国 1高虹 1张传生 1魏影允 1薛延 2劳为德1
作者信息
- 1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
- 2. Department of Biochemistry, Beijing Jishuitan Hospital, Peking University, Beijing 100035, China
- 折叠
摘要
Abstract
Background To explore the effect of human osteopontin (hOPN) on the proliferation, transmigration and expression of matrix metallproteinase-2 (MMP-2) and matrix metallproteinase-9 (MMP-9) in osteosarcoma (OS) cells in vitro. Methods The prokaryotic-expression vector of hOPN was produced, hOPN was then subcloned into E. coli BL21 (DE3) cells and purified with ProBondTM Columns. The proliferation, cell cycle and the expression of cyclin A in OS cells were investigated by using MTT assay, flow cytometry and Western blot respectively. The transmigration of OS cells was checked by using transwell cell culture chamber. The micro-pore-filter-membrane system was used to study the chemiotaxis of hOPN to OS cells. The levels of total protein were examined according to Coomassie Brilliant Blue manuals. The expression of MMP-2 and MMP-9 were evaluated by detecting the volume of degradation of gelatin on SDS-PAGE gel.Results The prokaryotic-expression vector of hOPN and purified hOPN protein were achieved hOPN promoted OS cells proliferation in a dose-dependent manner, and stimulated cyclin A expression in OS cells to accelerate cell division cycle, hOPN facilitated the trans-membrane migration of OS cells. hOPN also enhanced the secretion of MMP-2 and MMP-9 in OS cells. Conclusion hOPN could stimulate cyclin A expression in OS cells, hOPN has chemiotaxis to OS cells and increases their transmigration, hOPN enhances the secretion of MMP-2 and MMP-9 in OS cells.关键词
osteopontin/osteosarcona/proliferation/transmigration/matrix metallproteinaseKey words
osteopontin/osteosarcona/proliferation/transmigration/matrix metallproteinase分类
医药卫生引用本文复制引用
刘思金,胡国法,刘亚军,刘思国,高虹,张传生,魏影允,薛延,劳为德..Effect of human osteopontin on proliferation, transmigration and expression of MMP-2 and MMP-9 in osteosarcoma cells[J].中华医学杂志（英文版）,2004,117(2):235-240,6.
