昆虫学报2006，Vol.49Issue(2)：235-240,6.
三氟氯氰菊酯对棉铃虫神经细胞延迟整流钾电流的抑制作用
Inhibition effects of cyhalothrin on the delayed rectifier potassium current in the central neurons of Helicoverpa armigera
摘要
Abstract
The effects of cyhalothrin on the delayed rectifier potassium current in the central neurons of Helicoverpa armigera were studied using the patch clamp technique for the first time. The results showed that before application of cyhalothrin, 81% and 39% cells were activated at -30 mV and -40 mV (n = 21), respectively. In 15 min after cyhalothrin (10-5 mmol/L) application, 63% and 38% cells were activated at - 40 mV and - 50 mV ( n = 8),respectively. The amplitude of the current decreased significantly after application of cyhalothrin and the inhibition percentage reached 37.7% in 1 min (n = 19). After the application, activation curve shifted to the negative direction and the value of Vh changed significantly, but the k value did not change remarkably. In conclusion, the results suggest that under the action of cyhalothrin, the potassium channels can be activated more easily and current amplitude can be inhibited significantly, this is related to the nervous insensitivity, and potassium channels of Helicoverpa armigera central neurons are the action targets of pyrethroid insecticides.关键词
棉铃虫/钾通道/三氟氯氰菊酯/膜片钳/神经细胞/神经敏感性Key words
Helicoverpa armigera/potassium channel/cyhalothrin/patch clamp/neurons/nerve sensitivity分类
生物科学引用本文复制引用
王勇,贺秉军,伍春林,刘安西..三氟氯氰菊酯对棉铃虫神经细胞延迟整流钾电流的抑制作用[J].昆虫学报,2006,49(2):235-240,6.基金项目
国家自然科学基金资助项目(30100119) （30100119）
天津市自然科学基金资助项目(023614511) （023614511）
