国际眼科杂志2008，Vol.8Issue(12)：2359-2363,5.
胼酞嗪对眼血流和激光诱导的脉络膜新生血管及体外培养的内皮细胞的影响
Effects of hydralazine on ocular blood flow and laser-induced choroidal neovascularization
江伟 1邱春亿1
作者信息
- 1. 美国德州大学城德州农工大学健康科学中心医学院眼科药理研究所
- 折叠
摘要
Abstract
·AIM: To investigate the effect of hydralazine on choroidal blood flow in rabbits and laser-induced choroidal neovascularization (CNV) in rats and the effect of hydralazine on tube formation of human umbilical vein endothelial cells (HUVEC).used with raised intraocular pressure (IOP) of the left eye to 40mmHg.Hydralazine (10g/L) eye drops were instilled and ocular blood flow was measured with colored microspheres technique. Male Brown Norway rats were treated with Nd: YAG laser to break Bruch's membrane. Hydralazine (5, 10, 20g/L) eye drops or saline alone was instilled three times a day for 4 weeks after laser treatment. Fluorescein angiography (FA) and choroidal flat mount were used to measure the area of CNV. Tube formation of HUVEC was studied at different concen-trations of hydralazine.hydralazine eye drops enhanced the choroidal blood flow significantly at 30 and 60 minutes after drug instillation. After 4 week of drug treatment, 5, 10 and 20g/L hydralazine eye drops all reduced the CNV formation dramatically measured by fluorescein angiography and choroidal flat mount. When HUVEC was cultured on matrix gel for 48 hours, hydralazine at 3-30mg/L preven-ted the tube formation.vivo and HUVEC tube formation in vitro, and enhanced rabbits' choroidal blood flow after ischemia. It is hoped that hydralazine could be used to treat age-related ma-cular degeneration in the future.关键词
胼酞嗪/脉络膜/新生血管/人脐静脉内皮细胞/年龄相关性黄斑变性Key words
hydralazine/choroid/neovascularization/human umbilical vein endothelial cell/age-related macu-lar degeneration分类
医药卫生引用本文复制引用
江伟,邱春亿..胼酞嗪对眼血流和激光诱导的脉络膜新生血管及体外培养的内皮细胞的影响[J].国际眼科杂志,2008,8(12):2359-2363,5.
