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Crosstalk between angiotensin Ⅱ and platelet derived growth factor-BB mediated signal pathways in cardiomyocytes

中华医学杂志（英文版）2008，Vol.121Issue(3)：236-240,5.

Crosstalk between angiotensin Ⅱ and platelet derived growth factor-BB mediated signal pathways in cardiomyocytes

作者信息

1. Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China;Department of Physiology and Pathophysiology and Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education,Peking University Health Science Center,Beijing 100083,China
折叠

摘要

Abstract

Background Angiotensin Ⅱ(Angll) and platelet-derived growth factor(PDGF)-BB can induce hypertrophy in the cultured rat cardiomyocytes through different signal transduction pathways.Angll stimulates growth lhrough G protein coupled receptor(GPCR),while PDGF-BB acts via receptor tyrosine kinase(RTK).Although there has been much development On the individual Angll and PDGF-BB mediated signal pathways,little is known about the interactions between these two factors-Therefore,the crosstalk between Angll and PDGF.BB mediated signal pathways in the rat cardiomyocytes was investigated in this study.Metnods Primary culture of neonatal rat ventricular myocytes was prepared.The amount of tyrosine-phosDhorylated and non-phosphoryiated PDGF-β receptor,Gαq/11,and phospholipase C(PLC)β3 were measured by immunoblotting analysis. The statistical analysis was done by one-way ANOVA.Results Fyrosine-phosphorylated PDGF-β receptor was increased by 120.60% at 1 minute and recovered to the control level at 10 minutes after Angll stimulation.Phosphorylation of PDGF-β receptor triggered by Angll was blocked by losartan, a specific antagonist of AT1 receptor.PLC inhibitor U73122,protein kinase C(PKC)inhibitor staurosporine(STS) and mitogen-activated ERK activating kinase(MEK) inhibitor PD98059 also inhibited the Angll-induced phosphorylation of PDGF-β receptor.PDGF-BB slightly increased the expression of Gαq/11 protein.Conclusion Angll transactivates PDGF-β receptor via AT1 receptor-Gαq/11-PLC-PKC pathway in the rat cardiomyocytes.ERK also participates in the transactivation of PDGF-β receptor triggered by Angll.

关键词

angiotensin Ⅱ/platelet-derived growth factor-BB/G protein/platelet-derived growth factor-β receptor/ caratomyocytes

Key words

angiotensin Ⅱ/platelet-derived growth factor-BB/G protein/platelet-derived growth factor-β receptor/ caratomyocytes

分类

医药卫生

引用本文复制引用

..Crosstalk between angiotensin Ⅱ and platelet derived growth factor-BB mediated signal pathways in cardiomyocytes[J].中华医学杂志（英文版）,2008,121(3):236-240,5.

基金项目

This study was supported by the grants from the National Natural Science Foundation of China(No.30470630 and 30570715),theProgram for Changjiang Scholars and Innovative Research Team in University and National Basic Research Program of China ("973"Program,No.2007CB512004). （No.30470630 and 30570715）

中华医学杂志（英文版）

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ISSN：0366-6999

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