首页|期刊导航|南京医科大学学报(英文版)|Isolation and identification of proteins binding to the major breakpoint region(mbr) of bcl2 gene
南京医科大学学报(英文版)2009，Vol.23Issue(4)：236-240,5.
Isolation and identification of proteins binding to the major breakpoint region(mbr) of bcl2 gene
Isolation and identification of proteins binding to the major breakpoint region(mbr) of bcl2 gene
摘要
Abstract
Objective: We have previously found that mbr is a regulatory element of the bcl2 gene. The objective of this study is to isolate and identify the proteins binding to the 37 mbr in the 3 '-end of the mbr. Methods: Streptavidin magnetic particles were ligated to concatameric oligonucleofides of 37 mbr and incubated with the nuclear extracts of Jurkat cells. The DNA-binding proteins were eluted and then resolved by SDS-PAGE. After silver staining, the protein bands were excised and subjected to MALDI-TOF MS. Results: Several protein bands were detected after the isolation with magnetic particles, and Splicing factor, proline-and glutamine-rich(SFPQ), Poly(ADP-ribose)polymerase I(PARP), and promyelocytic leukemia protein(PML) were identified by MALDI-TOF MS. Conclusion: Several proteins were isolated and identified from the 37 mbr-protein complex. Results of this study establish a foundation for further study of the mechanisms by which mbr executes its regulatory function.关键词
bcl2 gene/37 mbr/DNA-binding proteinKey words
bcl2 gene/37 mbr/DNA-binding protein分类
医药卫生引用本文复制引用
Nan Yang,Yujie Sun,Changyan Ma..Isolation and identification of proteins binding to the major breakpoint region(mbr) of bcl2 gene[J].南京医科大学学报(英文版),2009,23(4):236-240,5.基金项目
This work was supported by grants from the National Natural Science Foundation of China(30500585) and the Natural Science Foundation of Jiangsu Province(BK2008450). （30500585）
