湖南农业大学学报（自然科学版）2009，Vol.35Issue(3)：237-241,5.
富硒酵母培养条件的优化
Condition optimization in cultivation of selenium-enriched yeast
摘要
Abstract
The study was made of fermentation conditions of selenium-enriched yeast. The following results were obtained by Single factor and orthogonal tests: the glucose was the optimal carbon source for yeast growth, and the optimal concentration of glucose was 4%～5%. The compound nitrogen which contained urea and peptone was the optimal nitrogen source for fermentation,and the optimal concentration of compound nitrogen were peptone 0.5% and urea 0.9%.The optimal concentration of yeast extract was 0.4%,and the optimal concentration of selenium was 25 μg/mL;the liquid volume of 30 mL in 250 mL flask at the speed of 200 r/min was the optimal condition for fermentation. The optimal initial pH for the strain growth was about 5～6. The optimal cultivation temperature was 28 ℃,and the fermentation could be finished in forty hours. Under the optimized conditions,the biomass (dry weight) reached 7.942 g/L and the total Se content of the cells was 16 486.48 μg /L.关键词
富硒酵母/发酵条件/优化Key words
selenium-enriched yeast/cultivation conditions/optimization分类
生物科学引用本文复制引用
陈里,詹逸舒,吴永尧..富硒酵母培养条件的优化[J].湖南农业大学学报（自然科学版）,2009,35(3):237-241,5.基金项目
湖南省科学技术厅重点项目(01NKY1002) （01NKY1002）
