辐射防护2009，Vol.29Issue(4)：237-242,6.
BEP2D细胞受照前后蛋白质组学研究
PROTEOME ANALYSIS FOR EFFECTS OF RADIATION ON BEP2D CELL
摘要
Abstract
The study analyzed the differential expression of proteins profile of the normal BEP2D cell and the BEP2D cell radiated with 1.5 Gy, 3 Gy γ rays by Proteomics technique. The results showed that approximately 1000 analyzed proteins per 2-D gel were isolated with molecular weights ranging between 14.4-94 kD and pI 3-10. Image analysis showed that irradiation with 1.5 Gy γ-rays contracting with control image caused down-regulation of 104 kinds of cell proteins and up-regulation of 15 proteins. Irradiation with 3 Gy γ-rays contracting with control image caused down-regulation of 144 kinds of cell proteins and up-regulation of 26 proteins. Irradiation with the two dimensional electrophoresis contracting with control image both caused down-regulation of 15 kinds of cell proteins and up-regulation of 3 proteins. Using mass spectrometry, a protein MLC was identified within the two protein spots (spot D and spot 660). All of this change of proteins expressing may result in the lung cancer,and the study can provide baisis for the study of lung cancer pathogenesis.关键词
蛋白质/组BEP2D细胞/肌球蛋白轻链/γ射线Key words
Proteomies, BEP2D Cell, MLC, γ Ray分类
能源科技引用本文复制引用
薛振伟,周小林,谷娟娟,蘧艳峰,崔梅萍..BEP2D细胞受照前后蛋白质组学研究[J].辐射防护,2009,29(4):237-242,6.基金项目
中国辐射防护研究院基础研究项目(项目编号:A0120060527-06). （项目编号:A0120060527-06）
