生命科学研究2000，Vol.4Issue(3)：237-242,6.
一种新型的棉花体细胞胚胎发生的快速诱导法
A Novel Method for Fast Induction of Cotton (Gossypiumhirsutum L.) Somatic Embryogenesis
摘要
Abstract
High frequency somatic embryogenesis was directly obtained from stem and leaf explants of cotton abnormal seedling. Direct induction of cotton somatic embryogenesis was affected by different concentrations and combinations of plant growth regulators. Modified MS medium supplemented with 0.1 mg/L IAA and 0.1 mg/L ZT was the best medium for direct induction of high frequency somatic embryogenesis from abnormal seedlings. With this medium, embryogenic callus was obtained within 10 days of culture, mature embryos was obtained within 20～30 days of culture, regenerative plant was obtained within 60 days of culture. For cotton somatic embryogenesis, there was no difference between stem and leaf explants, but difference existed among genotypes. This method was employed in cotton genetic transformation.关键词
陆地棉/异常苗/体细胞胚胎发生Key words
Gossypium hirsutum L./abnormal seedling/somatic embryogenesis分类
农业科技引用本文复制引用
周大云,张宝红,刘方,刘志红..一种新型的棉花体细胞胚胎发生的快速诱导法[J].生命科学研究,2000,4(3):237-242,6.基金项目
河南省“九五”攻关资助项目；河南省自然科学基金资助项目 （）
