中德临床肿瘤学杂志(英文版)2002，Vol.1Issue(4)：238-240,3.
表面抗原P43基因在异基因造血干细胞移植患者弓形虫感染诊断中的应用
Amplification of Surface Antigen P43 Gene and Its Application in Detection of Toxoplasma Gondii in Allogeneic Hematopoietic Stem Cell Transplantation
摘要
Abstract
Objective To establish a rapid, specific and sensitive diagnostic technique for the human Toxoplasma gondii infection in the recipients with allogeneic hematopoietic stem cell transplantation and discuss its clinical significance. Methods 30 patients undergoing allogeneic hematopoietic stem cell transplantation were detected by using ELISA and PCR.Results Among 30 recipients undergiong allogeneic hematopoietic stem cell transplantation , 3 were positive for Toxoplasma gondii antigen and 5 for surface antigen p43 gene with the positive rate being 13.3% and 16.67% respectively. 20 healthy people (negative for anti-Tox antibody) were also tested by using ELISA and PCR.Conclusion PCR is an accurate, relatively rapid, sensitive and specific method for detecting P43 gene of Toxoplasma gondii. Because PCR can be applied to a variety of different clinical samples, it can be considered as a valuable additional tool for identification of Toxoplasma gondii infections.关键词
异基因造血干细胞移植/弓形虫/PCR/ELISA/表面抗原P43基因Key words
allogeneic hematopoietic stem cell transplantation/toxoplasma gondii/polymerase chain reaction/ELISA/surface antigen P43 gene分类
医药卫生引用本文复制引用
周永安,余新炳,乔振华,陈观今..表面抗原P43基因在异基因造血干细胞移植患者弓形虫感染诊断中的应用[J].中德临床肿瘤学杂志(英文版),2002,1(4):238-240,3.基金项目
This project was supported by a grant from National Natural Sciences Foundation of China (No. 30070682). （No. 30070682）
