深圳大学学报（理工版）2008，Vol.25Issue(3)：238-243,6.
时间相关单光子计数记录快速荧光寿命图像
Recording fast time-series of fluorescence lifetime images by TCSPC FLIM
Bertram SU 1Vicky KATSOULIDOU1
作者信息
- 1. Becker & Hickl公司,Nahmitzer Damm 30,柏林D-12277,德国
- 折叠
摘要
Abstract
The acquisition time of TCSPC FLIM depends on the number of pixels of the image,on the required lifetime accuracy,and on the count rate available from the sample.For samples with high fluorophore concentrations,such as stained tissue or plant cells the available count rates may come close to the maximum counting capability of the currently used TCSPC FLIM techniques.We describe the behaviour of TCSPC at high count rates and estimate the size of counting loss and pile-up effects.It turns out that systematic lifetime errors are smaller than previously believed.TCSPC FLIM can therefore be used to record lifetime images within acquisition times of less than 1 s.To increase the recording of FLIM time series we employed a memory swapping technique that reads out the data of one recording step during the acquisition of the next one.With parallel operation of two TCSPC modules we were able to record FLIM time series at a rate of 2 images per second.We demonstrate the technique for the measurement of chlorophyll transients in live plant tissue.关键词
荧光寿命成像技术/时间相关单光子计数/叶绿素瞬态测量Key words
fluorescence lifetime imaging microscopy/time-correlated single photon counting/measurement of chlorophyll transients分类
生物科学引用本文复制引用
Bertram SU,Vicky KATSOULIDOU..时间相关单光子计数记录快速荧光寿命图像[J].深圳大学学报（理工版）,2008,25(3):238-243,6.
