首页|期刊导航|中华医学杂志（英文版）|Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay
中华医学杂志（英文版）2003，Vol.116Issue(2)：239-242,4.
Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay
Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay
摘要
Abstract
Objectives To investigate the DNA polymorphism of Sporothrix schenckii (S.schenckii) and to find the relationship between DNA patterns and geographic areas and clinical manifestations. Method The total DNA was extracted with hexadecyltrimethyl-ammonium bromide. Random A mplification of Polymorphic DNA (RAPD) assay was used to study DNA typing of 24 strains of S.schenckii collected from different areas and isolated from di fferent clinical types. Results Of seven random primers used, three primers (OPAA11, OPD18 and OPB07) gave good reactions, the sequences of which were 5'-ACCCGACCTG-3', 5'-GAGAGCCAAC-3', 5 '-GGTGAC～GCAG-3' respectively. The RAPD patterns of the 24 isolates were not completely identical, showing certain degrees of hereditary variability. Differ ent isolates showed a common conserved DNA band with the same primer. Different clinical types showed different genotypes. Conclusion RAPD analysis is useful in DNA typing of S.schenckii, the DNA band type of which is related to geographic origin and Clinical manifestation.关键词
Sporothrix schenckii/random amplification of polymorphic DNA assay/DNA typingKey words
Sporothrix schenckii/random amplification of polymorphic DNA assay/DNA typing分类
医药卫生引用本文复制引用
刘晓明,廉翠红,金礼吉,安利佳,杨国玲,林熙然..Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay[J].中华医学杂志（英文版）,2003,116(2):239-242,4.基金项目
This work was financially supported by a grant from the Education Commission of Liaoning Province (No. 990221069) （No. 990221069）
