四川大学学报（自然科学版）2001，Vol.38Issue(2)：239-242,4.
家兔中性粒细胞防御素的纯化及其对念珠菌抗菌活性的研究
IN VITRO ANTIFUNGAL ACTIVITY OF NEUIROPHH DEFENSINS AGAINST SOME CANDIDA Spp.
蒋献 1冉玉平 1黄宁 2吴琦 2周光平1
作者信息
- 1. 华西医科大学附属第一医院皮肤性病科,
- 2. 华西医科大学基础医学院感染免疫研究室,
- 折叠
摘要
Abstract
To investigate the role and mechanism of neutrophil defensins against some Candida spp. Neutrophil defensins were purified by polyacrylamide gel electrophoresis (SDS-PAGE), and neutrophil defensin NP2 were tested for its antifungal activity against C andida albican M1012R, NIH A-207, Candida kefyr, Candida tropicalis and Candida guilliermondii. NP2 was successfully isolated and purified by AU-PAGE, and demonstrated concentration-dependent killing of Candid albican M1012R, NIH A-207, Candida kefyr, Candida tropicalis and Candida guilliermondii in vitro. Acid urea polyacrylamide gel electrophoresis is simple and convient in isolating and purifying protein and polypeptides, neutrophil defensin NP2 exerted potent antifungal activity against Carndida spp.关键词
兔中性粒细胞防御素/念珠菌/抗菌活性分类
生物科学引用本文复制引用
蒋献,冉玉平,黄宁,吴琦,周光平..家兔中性粒细胞防御素的纯化及其对念珠菌抗菌活性的研究[J].四川大学学报（自然科学版）,2001,38(2):239-242,4.
