华西医科大学学报2001，Vol.32Issue(2)：240-242,3.
Leptin对人成骨细胞增殖和功能的影响
The Effects of Leptin on Proliferation and Function of Human Osteoblast
摘要
Abstract
Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and β-glycerophosphate. On the first, second and third days, the prolifertion of osteoblast， cultured with different concentrations of leptin， had no changes. The leptin-stimulated synthesis of osteocalcin of cells was found to be dose-dependent (P＜0.05), but not time-dependent (P＞0.05). Conclusion The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.关键词
成骨细胞/瘦素增殖/骨钙素分类
医药卫生引用本文复制引用
李双庆,赵红莉,魏松全,安振梅,谢秦,李晓佳,贾卫国..Leptin对人成骨细胞增殖和功能的影响[J].华西医科大学学报,2001,32(2):240-242,3.基金项目
四川省科委科研基金资助 （）
