中国当代儿科杂志2004，Vol.6Issue(4)：241-246,6.
内皮素及一氧化氮在肾小球硬化大鼠肾组织的表达及意义
Expression of endothelin and nitric oxide in the renal tissue of rats with glomerulosclerosis
摘要
Abstract
Objective It is not known whether endothdial factor-1 (ET-1) and nitric oxide (NO) are involved in glomerulosclerosis This study aimed at studying the changes and roles of ET-1 and NO in the process of glomerulosclerosis and the effects of the angiotensin converting enzyme inhibitor (ACEI), benazepril, and the angiotension Ⅱ receptor type Ⅰ antagonist, losartan. Methods Glomerulosclerosis was induced in rats by resecting one side of the kidneys and injecting adriblastine. The rats were randomly assigned into a glomerulosclerosis group (D group),a benazepril-treated glomerulosclerosis group (DB group), and a losartan-treated glomerulosclerosis group (DL group), 10per group. Ten rots were sham-operated (Control group) and were injected with normal saline into caudal veins. After 6weeks of benazepril or losartan administration, the mRNA expressions of ET-1 and iNOS in renal cortex were measured by reverse transcription polymerase chain reaction (RT-PCR). The protein levels of ET-1 and iNOS in renal cortex were detected by Western blotting, and the renal tissue Collagen Ⅳ and fibronectin were measured by immunohistochemistry.Results By the 4th week of adriblastine administration, urinary protein, serum cholesterol and blood urea nitrogen increased, while serum albumin decreased in Group D compared with those of the Control group (all P ＜0.05). RT-PCR and Western blotting demonstrated that the mRNA and protein expressions of ET-1 increased 3.58 and 2.83 times, and the mRNA and protein expressions of iNOS increased 4.28 and 3.15 times in the renal cortex of the Group D when compared with those of the Control group. The expressions of Collagen Ⅳ and fibronectin also significantly increased in Group D. After 6 weeks of benazepril or losartan treatment, the deposition of extracellular matrix in the Groups DB and DL was significantly reduced and mRNA and protein expressions of ET-1 and iNOS had decreased compared with those of the Group D. Meanwhile, the expressions of Collagen Ⅳ and fibronectin also decreased in the two treatment groups.Conclusions ET-1 and NO may participate in the process of glomerulosclerosis. Inhibition of ET-1 and iNOS blocks accumulation of extracellular matrix, and may avert glomerulosclerosis.关键词
肾小球硬化症/内皮素-1/诱导型一氧化氮合酶/大鼠Key words
Glomerulosclerosis/Endothelin-1/Inducible nitric oxide synthase/Rat分类
医药卫生引用本文复制引用
纪泽泉,黄翠雯,梁成结,陈波,陈盛强,孙卫文..内皮素及一氧化氮在肾小球硬化大鼠肾组织的表达及意义[J].中国当代儿科杂志,2004,6(4):241-246,6.基金项目
Guangzhou Committee of Science and Technology Key Program(1999-K-007-02). （1999-K-007-02）
