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DETECTION OF WHITE SPOT SYNDROME VIRUS(WSSV) OF PENAEUS CHINENSIS BY IN SITU HYBRIDIZATION

中国海洋湖沼学报（英文版）2000，Vol.18Issue(3)：241-246,6.

DETECTION OF WHITE SPOT SYNDROME VIRUS(WSSV) OF PENAEUS CHINENSIS BY IN SITU HYBRIDIZATION

作者信息

1. Aquaculture Research Laboratory, Ocean University of Qingdao, Qingdao 266003,China;Aquaculture Research Laboratory, Ocean University of Qingdao, Qingdao 266003,China;Aquaculture Research Laboratory, Ocean University of Qingdao, Qingdao 266003,China;Tokyo University of Fisheries, Konan, Minato, Tokyo, Japan
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摘要

Abstract

White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles, EcoR I-digested fragments of the WSSV genome were cloned; three of these fragments were used as non-radioactive probes labeled with DIG-11-dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV-infected tissues. The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.

关键词

WSSV/Penaeus chinensis/in situ hybridization

Key words

WSSV/Penaeus chinensis/in situ hybridization

分类

农业科技

引用本文复制引用

..DETECTION OF WHITE SPOT SYNDROME VIRUS(WSSV) OF PENAEUS CHINENSIS BY IN SITU HYBRIDIZATION[J].中国海洋湖沼学报（英文版）,2000,18(3):241-246,6.

基金项目

This work was supported by SRF for ROCS,SEM and the National 863 Project (Grant 819-Q-08)and Project under major State Basic Research Development Program(G1999012002). （Grant 819-Q-08）

中国海洋湖沼学报（英文版）

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ISSN：2096-5508

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