昆虫学报2005，Vol.48Issue(1)：24-30,7.
东亚飞蝗山西两地理种群酯酶特性的比较研究
Characterization and comparison of general esterases from two field populations of the oriental migratory locust, Locusta migratoria manilensis ( Meyen ) (Orthoptera: Acrididae) from Shanxi Province
摘要
Abstract
General esterases in two field populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen),respectively from Yongji and Linyi, Shanxi Province, were characterized and compared. No distinct difference was detected in banding patterns of general esterases between the two populations, when α-naphthyl acetate was used as substrate in nondenaturing polyacrylamide gel electrophoresis ( PAGE). Nevertheless, the general esterase activities in the Yongji population were 1.81- and 1.20-fold higher, respectively, than those of the Linyi population when α-naphthyl acetate and α-naphthyl butyrate were used as substrates. Increased esterase activity in the Yongji population might be correlated to several additional esterase bands detected with non-denaturing PAGE. Inhibition studies showed that 84.94% and 91.47 % of esterases were Btype in Yongji and Linyi population, respectively. The Linyi population appeared to be more susceptible to malaoxon than the Yongji population. We estimated that such differences might be attributed to the different control practices for locusts in the two areas.关键词
东亚飞蝗/酯酶/酶动力学/酯酶抑制/抗性Key words
Locusta migratoria manilensis/esterase/enzyme kinetics/esterase inhibition/resistance分类
生物科学引用本文复制引用
刘新,贺艳萍,马恩波..东亚飞蝗山西两地理种群酯酶特性的比较研究[J].昆虫学报,2005,48(1):24-30,7.基金项目
国家自然科学基金项目(30170612) （30170612）
国家自然科学基金国际合作项目(30210103147) （30210103147）
山西省科技厅国际合作项目(041005) （041005）
