中国临床康复2005，Vol.9Issue(19)：243-245,3.
骨保护素抑制肿瘤坏死因子诱导的成骨细胞凋亡
Inhibitory effect of osteoprotegerin on tumor necrosis factor-induced apoptosis in osteoblastic cells
摘要
Abstract
BACKGROUND: Osteoporosis involves two aspects, osteoblast apoptosis and osteoclast differentiation and maturation. The apoptotic effect of tumor necrosis factor-α varies in different cell lines and it remains unclear whether osteoprotegerin has inhibitory effects on the apoptosis of osteoblastic cells.OBJECTIVE: To study the interaction between tumor necrosis factor-α and osteoprotegerin on the apoptosis of osteoblastic cells.DESIGN: Observational comparative study based osteoblasts and MG63 cells as subjects and murine WEHI 164 cells as positive controls.SETTING: Institute of Oral Medicine, General Hospital of Chinese PLA, and Veterans' Affairs Medical Center, Pittsburgh.MATERIALS: The experiment was conducted in the Dr. Blair' s Laboratory and Oral Institute from January 2001 to December 2003. Commercial cell group, RPMI 1 640 culture medium and various related proteins were selected in this study.METHODS: Osteoblasts were differentiated from human mesenchymal stem cells in the culture medium. and were ready for the study on apoptosis with MG63. Annexin V and TUNEL analysis were used to assay osteoblasts'apoptosis induced by tumor necrosis factor-α.MAIN OUTCOME MEASURES: The number of total cells and apoptotic cells.RESULTS: As FasL induced apoptosis, tumor necrosis factor-α could obviously induce the apoptosis of MG63 osteogenic cells, mesenchymal stem cells and osteoblasts in concentration-and time-dependent manner. Cells showed obvious apoptosis after 2 -4 hours' effect by tumor necrosis factor-α in low concentration ( 170 - 500 pmol/L); osteoprotegerin, on the other hand, at 0. 45 - 1.5 nmol/L inhibited the apoptosis induced by tumor necrosis factor-α of 500 pmol/L. Osteoblasts could secrete osteoprotegerin, whereas osteoclasts and precursor of osteoclasts could produce tumor necrosis factor-α and work together to reduce the apoptosis of osteoblasts.CONCLUSION: Tumor necrosis factor-α can effectively induce the apoptosis of osteoblastic cells while osteoprotegerin has the ability to inhibit it. The mechanism of osteoprotegerin on osteoporosis involves at least two aspects, by inhibiting the maturation of osteoclasts and the apoptosis of osteoblasts.关键词
骨质疏松/肿瘤坏死因子/脱噬作用分类
医药卫生引用本文复制引用
步荣发,李亚男,Jennifer I Oakley,Harry C Blair..骨保护素抑制肿瘤坏死因子诱导的成骨细胞凋亡[J].中国临床康复,2005,9(19):243-245,3.基金项目
国家自然科学基金专项基金项目(30340052) （30340052）
美国国立卫生研究院部分资助课题(AG12951,AR47700) （AG12951,AR47700）
