棉花学报2008，Vol.20Issue(4)：243-248,6.
瑟伯氏棉和异常棉的陆地棉导入系的EST-SSR和gSSR分析
Characteristics of Gossypium thurberi and G.anomalum Introgression Lines of G.hirsutum Revealed by EST-SSR and gSSR
摘要
Abstract
Eight introgression lines (ILs) of G.thurberi and G.anomalum were analyzed using EST-SSR and gSSR. The results showed that the bands detected by SSR markers could be sorted into two classes: (I) no differences between the eight ILs and Jinmian 6, the upland cotton receptor, and (II) differences among the eight ILs and Jinmian 6. In class I, five sub-classes can be identified. In class II, four sub-classes could be identified for EST-SSR: (1) bands detected in Jinmian 6 but absent in some ILs; (2) band size changed compared to Jinmian 6; (3) bands that introgressed from G.thurberi and G.anomalum while bands same as Jinmian 6 disappeared in some ILs; (4) the novel bands that differ from either the donor or the receptor appeared in some ILs, while bands size changed compared to Jinmian 6 in some other ILs. For gSSR, two additional sub-classes could be identified: (5) additional bands appeared in some ILs; (6) both donors′ and Jinmian 6′s bands appeared in ILs. The appearance of novel bands or disappearance of parental bands may result from the changes of SSR region and flanking sequence. Compared to the gSSRs, EST-SSRs were more variable between the ILs and Jinmian 6, which may be a result of the introgressed good fiber quality of the ILs.关键词
导入系/瑟伯氏棉/异常棉/EST-SSR/gSSRKey words
introgression lines/G.thurberi/G.anomalum/EST-SSR/gSSR分类
农业科技引用本文复制引用
林忠旭,王锦峰,张献龙..瑟伯氏棉和异常棉的陆地棉导入系的EST-SSR和gSSR分析[J].棉花学报,2008,20(4):243-248,6.基金项目
国家973计划(2004CB117301) （2004CB117301）
