中国免疫学杂志2001，Vol.17Issue(5)：245-248,4.
TCRVβ基因亚家族的优势取用和PTK信号传导途径的激活及体外对肝癌细胞凋亡的诱导
The usage of TCRVβ gene and activation of PTK signal pathway in McAb costimu-lated PBLs and induction of in vitro apoptosis of hepatoma cells
黄树林 1周继斌 1陈维春 1林月霞 1邓涛 1罗利琼 1肖兰凤 1杨红1
作者信息
摘要
Abstract
Objective: To study the expression of TCRVβ subfamily which specially recognize the hepatoma cell antigen and the apoptosis of hepatoma cell induced by McAb costimulated PBLs.Methods: The change of the phenotype of PBLs was studied by flow cell cytometry and the level of the expression of TCRVβ was studied by RT-PCR and Southern blot,the PTX by westem blot. The hypermicroscopic ultrastructure was ob served through transmission electron microscope. Results: The level of CD3 and CD8 of PBLs was significantly increased after acted with hepatoma cells, while there was no change in CD4. The expression of TCRVβ7of PBLs was dramaticly increased and peaked at 4 days. PTK in creased correspondently, to 58% compared with 11% in control. Besides anti-CD3 McAb induced lymphocyte apoptosis, the mediated apoptosis of hepatoma cells was found in the other three groups. Conclusion: TCRVβ7was the tumor antigen specific T cell receptor, and it activate the PTK signal pathway.The McAb activated lymphocytes initiated apoptosis in hepatoma cells.关键词
凋亡共刺激TCRVβ基因PTX分类
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黄树林,周继斌,陈维春,林月霞,邓涛,罗利琼,肖兰凤,杨红..TCRVβ基因亚家族的优势取用和PTK信号传导途径的激活及体外对肝癌细胞凋亡的诱导[J].中国免疫学杂志,2001,17(5):245-248,4.
