南京医科大学学报(英文版)2005，Vol.19Issue(5)：247-250,4.
Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium
Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium
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作者信息
- 1. Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Neurology,the affiliated Union Hospital of Tongji Medical Collage,Huazhong University of Science and Technology,Wuhan 430022,China
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摘要
Abstract
Objective: To explore the protective effect of minocycline on the apoptosis of cellular parkinsonism models induced by MPP+ . Methods: Using PC12 cells as the apoptotic model of dopaminergic neurons, MC and MPP+ were added into the culture medium of PC12 cells, and using MTT to assay the cell viability and metabolic state; The cells apoptosis was assayed by electrophoresis method and using flow cytometry FACS to assay the apoptosis ratio. Results: Added the MPP+ to get the concentration of 10μmol/L, the cellular parkinsonism model of apoptosis had been prepared. The pre-treatment of MC (100 μmol/L) could significantly increase the PC12 cell viability. The apoptosis ratio of MC + MPP+ group was significantly lower than that of MPP+ group, but was still significantly higher than that of control group. Conclusion: MC may protect the cell apoptosis induced by MPP+ to some extent.关键词
minocycline/PC12 cell apoptosis/parkinson disease/1-methyl-4-mhenylpyridiniumKey words
minocycline/PC12 cell apoptosis/parkinson disease/1-methyl-4-mhenylpyridinium分类
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..Minocycline protects the apoptosis of PC12 cells induced by 1-methyl-4-phenylpyridinium[J].南京医科大学学报(英文版),2005,19(5):247-250,4.
