首页|期刊导航|南京医科大学学报(英文版)|Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells
南京医科大学学报(英文版)2009，Vol.23Issue(4)：247-250,4.
Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells
Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells
摘要
Abstract
Objective: To investigate the expression of OX40 ligand(OX40L) on C-reactive protein(CRP)-triggered mouse aorta endothelial cells (MAECs) in vitro. Methods: MAECs from aorta were isolated by digestion with collagenase type Ⅱ. The cell growth was confirmed by morphological characteristics and the immunological marker, factor Ⅷ(or Willebrand factor, vWF). The expression of OX40L by MAECs was detected by RT-PCR and western blot after incubating with 100 μg/ml CRP for 48 hours. Results: Twenty-day cultures of MAECs formed confluent monolayer of a cobblestone pattern. RT-PCR and western blot assay showed that the level of OX40L expression in MAECs receiving CRP treatment was higher than control. Conclusion: A reliable method is described to isolate and propagate MAECs. CRP upregulates OX40L expression in MAECs.关键词
endothelial cells/C-reactive protein/OX40LKey words
endothelial cells/C-reactive protein/OX40L分类
医药卫生引用本文复制引用
Fei Wang,Shushu Zhu,Xuefu Han,Jindan Xu,Jinnan Zhang,Di Yang..Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells[J].南京医科大学学报(英文版),2009,23(4):247-250,4.基金项目
This work was supported by National Natural Science Founda-tion of China(30770894) and Medical Foundation key scholar ofJiangsu Province(RC2007037). （30770894）
