中国病理生理杂志2001，Vol.17Issue(3)：248-250,3.
L-精氨酸对大鼠血管损伤后内膜增生
及相关细胞周期调控基因表达的影响
Effect of L-arginine on intimal proliferation and expression of related cell
cycle regulatory factors after vascular injury in rats
聂晓敏 1刘瑞云 2黄振文 2董建增 2张金盈 1李庚山1
作者信息
- 1. 湖北医科大学附属第一医院心内科,
- 2. 河南医科大学第一附属医院心内科,
- 折叠
摘要
Abstract
AIM: To investigate the effect of L-arginine (L-Arg) on intimal proliferation and expression of related cell cycle regulatory factors after vascular injury in rats. METHODS: Rats were divided into three groups :sham operation group, balloon injury group(this group included balloon 48 h,7 d and 14 d subgroup) and balloon+L-Arg group. Neointima area were calculated morphologiocally. The expression of cyclin dependent kinase-2(CDK2),cyclin E and proliferation cell nuclear antigen (PCNA) were measured by means of immunohistochemical technique and computer image analyzer. RESULTS: After vascular balloon injury, the level of plasma NO decreased, CDK2、cyclin E and PCNA expressed in the media at 48 h and in the neointima at 7 d and 14 d but with low and undetected expression in the media, the expression of CDK2, cyclin E and PCNA increased with the intima thickening. Compared with balloon 14 d group, the plasma NO level increased (P＜0.01）, the neointima area reduced by 59.1%(P＜0.01) and the positive expression indexes of CDK2, cyclin E and PCNA decreased by 36.1%, 46.3% and 76.2% respectively in balloon+L-Arg group (P all＜0.01）. CONCLUSION: L-Arg can effectively repress intima proliferation after vascular injury, which may be associated with its inhibiting the proliferation of vascular smooth muscle cell through downregulating the excessive expression of CDK2, cyclin E and PCNA.关键词
精氨酸/血管成形术,气囊/细胞周期`分类
医药卫生引用本文复制引用
聂晓敏,刘瑞云,黄振文,董建增,张金盈,李庚山..L-精氨酸对大鼠血管损伤后内膜增生
及相关细胞周期调控基因表达的影响[J].中国病理生理杂志,2001,17(3):248-250,3.
