首页|期刊导航|中国海洋湖沼学报（英文版）|Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila
中国海洋湖沼学报（英文版）2008，Vol.26Issue(3)：248-255,8.
Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila
Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila
摘要
Abstract
Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension at a density of 5.2 × 106 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution (0.85%, w/v). Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection (dpi) for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase (SOD) activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.关键词
Rana catesbeiana/Aeromonas hydrophila/intraperitoneal injection/non-specific immune responseKey words
Rana catesbeiana/Aeromonas hydrophila/intraperitoneal injection/non-specific immune response分类
海洋科学引用本文复制引用
..Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila[J].中国海洋湖沼学报（英文版）,2008,26(3):248-255,8.基金项目
Supported by National High-Tech Research and Development Program of China (863 Program, No.2001AA5070, 2002AA639600) and Natural Science Foundation of Fujian Province of China (No.B0410022, 2006F5066) （863 Program, No.2001AA5070, 2002AA639600）
