农业生物技术学报2006，Vol.14Issue(2)：250-254,5.
转基因产品中PAT蛋白的酶联免疫检测
Enzyme Linked Immunosorbent Assay for PAT Protein Detection in Genetically Modifed Rape
摘要
Abstract
The immunoassay method to detect genetically modified (GM) rape (Brassica campestris ) containing the phosphinothricin acetyltransferase (PAT) were developed and applied. The purified PAT was identified by Western blot and enzymic activity analysis. The polyclonal antibody against purified PAT protein was obtained and purified by both saturated ammonium sulfate method and protein A-Sepharose 4B. The sensitivity and cross-reactivity of polyclonal antibody has been demonstrated in ELISA test.The result of the ELISA for antiserum sensitivity was about 2× 10-5 mg/mL and the cross-reactivity determined experimentally showed a high degree of specificity for the antiserum used, because values were all less than 0.1%. The detections of transgenic plants were evaluated with two transgenic rapes (MS1/RF1 and MS8/RF3) and the transgenic rapes (MS1/RF1 and MS8/RF3) could be easily distinguished by ELISA.关键词
酶联免疫检测技术/PAT蛋白/转基因产品检测Key words
ELISA/PAT/GMO detection分类
农业科技引用本文复制引用
许文涛,黄昆仑,邓爱科,罗云波..转基因产品中PAT蛋白的酶联免疫检测[J].农业生物技术学报,2006,14(2):250-254,5.基金项目
Foundation item: The state High Science and Technology Program(863) project (No.2001AA212291) of China and "948" Project (No. 2005-Z32)of China. （863）
