中国病理生理杂志2002，Vol.18Issue(3)：250-255,6.
肝素增强佛波酯对人鼻咽癌CNE2细胞的作用:调c-jun,p5 3,p21的表达
Potentiation of the action of phorbol ester by heparin in human CNE2 cells: Association with enhanced expression of c-jun,p53 and p21
摘要
Abstract
AIM: To measure the effect of addition of hep arin to TPA on cell proli feration and apoptosis in CNE2 cells and investigate the possible molecular mech anisms underlying heparin and TPA interaction on cell proliferation and apoptosi s. METHODS: Cell viability and cell cycle were determined by cel l counting and flow cytometry. Apoptosis was evaluated by terminal deoxynucleotidyl transferase -mediated dUPT nick-end labeling (TUNEL) and agarose gel electrophoresis. The ex pression of c-jun,c-fos,p21 and p53 was examined by Western blot. RESU LTS: TPA alone inhibited CNE2 cell proliferation and evoked apoptosis associated with ty pical morphological changes and DNA fragmentation,which was augmented when hepa rin was added. Compared with TPA or heparin alone,TPA plus heparin obviously en hanced the number of TUNEL-positive cells from 23%±1.2% to 51%±0.9%. After exp os ure to different concentrations of heparin (with or without TPA) for 24 h,CNE2 cells were accumulated G 0/G 1 phase. There was a decrease in the number of ce lls in S phase by the combined heparin and TPA treatment compared to heparin or TPA alone. Western blot analysis revealed that TPA induced the increases in c-jun an d p53,p21 protein expression and the levels were remarkably increased following heparin in combination with TPA treatment,whereas no significant change in c-f os was detected. CONCLUSION: These results suggest that heparin synergistically potentiates the action of TPA in CNE2 cells,which may be associated with the in crease in c-jun protein level and the upregulation of p21,p53 protein expressio n.关键词
肝素/细胞周期/细胞凋亡/佛波醇酯类Key words
Heparin/Cell cycle/Apoptosis/Phorbol esters分类
医药卫生引用本文复制引用
李红良,陈丹丹,张海伟,钟玲,李小红,罗英儒,任先达,司徒锐..肝素增强佛波酯对人鼻咽癌CNE2细胞的作用:调c-jun,p5 3,p21的表达[J].中国病理生理杂志,2002,18(3):250-255,6.基金项目
The Oversas Chinese Affairs Office of the State Counc il Foundation (No. 98-33) （No. 98-33）
National Natural Science Foundation of China (No. 3950 0056) （No. 3950 0056）
