河南医科大学学报2001，Vol.36Issue(3)：251-253,3.
心肌M2胆碱受体抗体对大鼠心房及心室肌cAMP含量的影响
Effects of anti-peptide antibody against human M2 muscarinic receptors on the cAMP content in rat ventricles
摘要
Abstract
Aim:To study the effects of anti-peptide antibodies(M2-Ab) against the second extracellular loop of human muscarinic receptor 2 on the cAMP content in rat atria and ventricles.These effects were compared with those of the muscarinic receptor agonist carbachol (Carb).Methods:Radioimmunoassay technique,was used for determining the effects of anti-pepitide antibodies and carb on cAMP content in rat ventricles.Result:①both Carb and M2-Ab were able to inhibit the isoproterenol (Iso) stimulated cAMP production in rat atria and ventricles.Carb at 2μmol/L,10μmol/L and 50μmol/L decreased Iso-stimulated cAMP production by (8.5±1.2)%，(16.2±1.4)% and (29.5±2.1)%，respectively;whereas M2-Ab at 50nmol/L,100nmol/L and 400nmol/L decreased it by (6.1±0.6)%,(17.3±1.8)% and (31.7±3.1)% (P＜0.01),respectively.②Both Carb and M2-Ab could also inhibit the basal cAMP content significantly.Carb at 10μmol/L and M2-Ab at 100nmol/L decreased it by (49.2±4.3)% and (64.3±5.1)%,respectively.③The inhibitory effects of both M2-Ab and Carb on Iso-stimulated cAMP production were significantly prevented by atropine at 1.5μmol/L.The inhibitory effect of M2-Ab at 100nmol/L was almost completely abolished by the peptide (700 nmol/L) itself.Conclusion:The anti-M2 receptor antibodies display a stimulatory muscarinic activity similar to muscarinic receptor agonist,carbachol,in inhibiting isoproterenol stimulated cAMP production of ventricles.关键词
抗肽抗体/M2胆碱受体/cAMP分类
医药卫生引用本文复制引用
朱琳,李香珍,冯羡菊,王文泽,张琳,陈爱莲..心肌M2胆碱受体抗体对大鼠心房及心室肌cAMP含量的影响[J].河南医科大学学报,2001,36(3):251-253,3.基金项目
国家自然科学基金资助项目 39370304 （）
