中国肿瘤生物治疗杂志2000，Vol.7Issue(4)：251-254,4.
联合应用bcr-abl融合基因反义寡核苷酸与c-myb基因反义寡核苷酸对K562细胞作用的研究
The Effect of Combining bcr-abl Antisense Phosphorothioate Oligodeoynucleotides and c-myb Aspo on K562 Cell Line
摘要
Abstract
Objective: To study the effect of combining bcr-abl Aspo and c-myb Aspo on K562 cells. Methods: Cellswere exposed to oligomers. Cell inhibitory rate was determined by typan blue dye exclusion. CFU-K562 cells were culturedin 0.8% methyleellulose. P210 was measured by flow cytometry. Cellular bcr-abl mRNA was detected by RT-PCR semiquantitative analysis. Cell apoptosis was measured by flow cytometry and observed by electron microscope. Results: When the concentration of both bcr-abl Aspo and c-myb Aspo was 5 μmol/L, K562 cells were still growth in clone state. The growth inhibitory rate was 61.7% at 120 h. P210 was depressed at 24 h and went up to 25.7% at 120 h. The apoptosis rate was 22.5%. While K562 cells were dealt with 10 μmol/L bcr-abl Aspo and 10 μmol/L c-myb Aspo, the cells were growth in dispersal. The cell growth inhibitory rate reached to 92.2% and 64.3% of K562 cells were induced to apoptosisat 120 h. P210 was complelely depressed untill 120 h. The decrease of bcr-abl mRNA was from 69.2% to 85.3% after incubation 48 h with 5 μmol/L Aspo and 10 mol/L. Conclusion: It emerges coordination to combine bcr-abl Aspo and c-myb Aspo on K562 cells, and enhances the anti-leukemia effect.关键词
反义寡核苷酸/bcr-abl融合基因/c-myb基因/K562细胞系Key words
antisense oligonucleotides/bcr-abl/c-myb/K562 cell line分类
生物科学引用本文复制引用
石奇珍,吕联煌..联合应用bcr-abl融合基因反义寡核苷酸与c-myb基因反义寡核苷酸对K562细胞作用的研究[J].中国肿瘤生物治疗杂志,2000,7(4):251-254,4.基金项目
本课题受福建省科委优先发展基金(98-Z-213)资助 （98-Z-213）
