首页|期刊导航|南京医科大学学报(英文版)|The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells
南京医科大学学报(英文版)2009，Vol.23Issue(4)：251-256,6.
The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells
The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells
摘要
Abstract
Objective: This study investigated the effects of rh-TRAIL with or without chemotherapeutic drugs on the apoptosis of the osteosa-rcoma cell line, MG-63, and the influence of chemotherapeutic drugs on changes in the expression of DR5 and YinYang 1(YY1) in MG-63 cells. Methods: The effects of treatment with rh-TRAIL alone and/or chemotherapeutic drugs on MG-63 cell growth inhibition and apoptosis were measured using the MTT assay, FACS analysis of Annexin Ⅴ labeled cells, and the mRNA changes of DR5 and YY1 were detected by RT-PCR. Results: Rh-TRAIL protein inhibited the growth of MG-63 cells, and this inhibition was increased by adriamycin and IFN-γ. Adriamycin and IFN-y significantly facilitated the induction of the expression of DR5 and reduced the expression of YY1. Conclusion: The apoptosis-inducing effect of rh-TRAIL in MG-63 ceils was enhanced by chemotherapeutic drugs.关键词
TRAIL/IFN-γ/adriamycin/osteosarcoma cells/cell apoptosisKey words
TRAIL/IFN-γ/adriamycin/osteosarcoma cells/cell apoptosis分类
医药卫生引用本文复制引用
Chao Deng,Zengwu Shao,Xiaoqian Xiong,Zhiehuan Liu,Zhicai Zhang..The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells[J].南京医科大学学报(英文版),2009,23(4):251-256,6.基金项目
This work was supported by National Nature Science Foundation of China(30700841). （30700841）
