生物技术通报Issue(2)：25-27,3.
用天冬酰胺代替谷氨酰胺培养产尿激酶原CHO工程细胞
Substitution of Asparagine for Glutamine for the Cultivation of
Recombin ant CHO Cell Line Producing Prourokinase
陈昭烈 1刘红 1吴本传 1李世崇 1林福玉1
作者信息
- 1. 军事医学科学院生物工程研究所, 北京 100071
- 折叠
摘要
Abstract
Based on the effects of asparagine on cell growt h and prourokinase ex pression of a recombinant CHO cell line CL-11G, the feasibility of using asparag ine as another nitrogen source substitute for glutamine for animal cell culture media was assessed. It was found that the spontaneous decomposition of free from asparagine was much more slow than that of glutamine, and asparagine could supp orted the cell growth and prourokinase expression of CL-11G as efficent as that of glutamine. The results show that using asparagine instead of glutamine is in favor of lessening the concentration of ammonia from spontaneous decomposition o f glutamine.关键词
细胞培养/CHO工程细胞/尿激酶原/氨Key words
cell culture/recombinant CHO cells/prouroki nase/ammonia分类
生物科学引用本文复制引用
陈昭烈,刘红,吴本传,李世崇,林福玉..用天冬酰胺代替谷氨酰胺培养产尿激酶原CHO工程细胞[J].生物技术通报,2001,(2):25-27,3.
