花生学报2005，Vol.34Issue(4)：25-27,3.
花生MADS-box 基因片段的克隆
Cloning of a Partial MADS-box Gene in Groundnut (Arachis hypogaea L.)
摘要
Abstract
MADS-box genes are the key genes involved in growth and development regulation in plants. Based on alignment of MADS-box genes, degenerate primers were designed and used to amplify an expected fragment with 130 bp size from genomic DNA of cultivar JL24. The results of sequences and alignment revealed that the fragment showed highly homology ranging from 79% to 84% to 26 MADS-box genes. The results indicated that a partial MADS-box gene was cloned successfully from cultivated groundnut.关键词
MADS-box基因/基因克隆/花生Key words
MADS-box/gene cloning/peanut分类
农业科技引用本文复制引用
袁美,K K Sharma,李双铃,陶海腾,任艳,禹山林..花生MADS-box 基因片段的克隆[J].花生学报,2005,34(4):25-27,3.基金项目
山东省自然科学基金项目(Q2003D02)This work was done at the Genetic Transformation Laboratory of ICRISAT, and supported in part by natural science fund of Shandong province and Harvest Plus Global Challenge Program on Biotortification. （Q2003D02）
