中国当代儿科杂志2001，Vol.3Issue(1)：25-27,3.
成年大鼠骨髓基质细胞体外诱导分化为NSE阳性细胞
Differentiation of Adult Rat Bone Marrow Stromal Cells Into NSE-positive Cells
摘要
Abstract
Objective To study the protocol that induces adult rat bone marrow stromal cells (MSCs) to express neuron-specific enolase (NSE) in vitro. Methods MSCs were preinduced with β-mercaptoethanol for 24 h, and then induced for 5 h. The positive percentages of NSE protein expression were measured by immunocytochemistry SABC staining. Results After the induction, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and processes which formed extensive networks. The positive percentage of NSE protein expression was (63.7±4.5)%. Conclusions β-mercaptoethanol may induce adult rat MSCs to NSE-positive cells in vitro.关键词
骨髓基质细胞/神经细胞特异性烯醇化酶/β-巯基乙醇/大鼠分类
生物科学引用本文复制引用
贾延,杨于嘉,宋元宗..成年大鼠骨髓基质细胞体外诱导分化为NSE阳性细胞[J].中国当代儿科杂志,2001,3(1):25-27,3.
