中国农业科学（英文版）2002，Vol.1Issue(1)：25-29,5.
Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle
Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle
XU Qiong-fang 1LI Lian-cheng 1CHEN Xiao 1TIAN Fang 1MA You-zhi 1YE Xing-guo 1ZHANG Zeng-yan 1XU Hui-jun 1XIN Zhi-yong1
作者信息
- 1. Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081
- 折叠
摘要
Abstract
The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with theDNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.关键词
Galanthus nivalis agglutinin/bar gene/Particle bombardment/Transgenic wheatKey words
Galanthus nivalis agglutinin/bar gene/Particle bombardment/Transgenic wheat引用本文复制引用
XU Qiong-fang,LI Lian-cheng,CHEN Xiao,TIAN Fang,MA You-zhi,YE Xing-guo,ZHANG Zeng-yan,XU Hui-jun,XIN Zhi-yong..Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle[J].中国农业科学（英文版）,2002,1(1):25-29,5.
