眼科学报2004，Vol.20Issue(1)：25-29,5.
Characteristics of Optic Nerve Damage Induced by Chronic Intraocular Hypertension in Rat
Characteristics of Optic Nerve Damage Induced by Chronic Intraocular Hypertension in Rat
摘要
Abstract
Purpose:To set up the Sharma's chronic intraocular hypertension model and investigate the intraocular pressure (lOP) as well as the optic nerve damage of this model in rat.Methods:The operations of the chronic intraocular hypertension model were performed as described by Sharma in 60 male Lewis albino rats. IOP was measured using the TonoPen XL immediately after surgery and then at 5 day, 2 week or 4 week intervals. Cresyl violet staining of whole-mounted retinas was used to label retinal ganglion cells (RGCs),then RGCs were counted. Paraphenylenediamine (PPD) staining was performed in the semi-thin cross sections of optic nerve of rat, in order to know whether the axons of optic nerve were degenerated or not. Results:There were 47 rats with higher IOP after the episcleral veins cauterized in 60rats. The ratio of elevated IOP was 78.3%. The IOPs were stable in 4 weeks. After cresyl violet staining, the RGCs loss was 11.0% and 11.3% was found in the central and peripheral retina respectively after 2 weeks of increased IOP. After 4 weeks of increased lOP, the loss of RGCs was 17% for the central retina and 24.6% for the peripheral retina. In the retinas without higher IOP, there was no loss of RGCs. PPD staining showed that optic nerve of rat with about 5.3% damage of axons located at the superior temporal region. Region of affected optic nerve 1 mm posterior to the globe by light microscope showed evidence of damaged axons with axonal swelling and myelin debris.Conclusion:Sharma's chronic intraocular hypertension model is a reproducible and effective glaucoma model, which mimics human glaucoma with chronically elevation IOP and induced RGCs loss and damage of optic nerve. Eye Science 2004;20:25-29.关键词
intraocular hypertension/animal model/retinal ganglion cells (RGCs)/optic nerve damageKey words
intraocular hypertension/animal model/retinal ganglion cells (RGCs)/optic nerve damage引用本文复制引用
Jiantao Wang,Jian Ge,A.A. Sadun,T.T. Lam..Characteristics of Optic Nerve Damage Induced by Chronic Intraocular Hypertension in Rat[J].眼科学报,2004,20(1):25-29,5.基金项目
This project was supported by Natural Scientific Foundation(No: 3017100) and a key project grand of the Science and Technology of Guangdong Province (No: 2KM 052028). （No: 3017100）
