生命科学仪器2007，Vol.5Issue(1)：25-31,7.
线粒体ATP合成酶复合体分离纯化的方法学研究与探讨
Discussion and Practice on the Improved Methods for Isolation and Purification of Mitochondria ATP Synthase Complex
摘要
Abstract
In the past 50 years,scientists have done so much on ATP synthase's structure and its function alreadv,but people known function more and the mechanism fewer,and there are much more work need to do.High purified ATPase and subunits which should come firstly are the key factors in study of enzyme's structure and catalvtic mechanism.This review has summarized some valuable methods in extraction and purification of ATP synthase through compared with kinds of methods came from different workgroups all of the world,and mainly introduced the isolation of bovine heart mitochondria.submichondrial particles,F1-ATPase.F0-AIPase and their subunits such as a,b,g,d,e in F1-ATPase and b,c,d,e,f,g,h,F6,A6L in FO moiety and oligomycin sensitive conferring ATPase (OSCP) and the ATPase inhibitor (IF1) in details.关键词
牛心线粒体/亚线粒体/FO-ATPase/F1-ATPase/亚基Key words
Bovine Heart Mitochondria,Submitochondria,FO-ATPase,F1-ATPase,Subunits分类
生物科学引用本文复制引用
朱杰,张斌,贺道华,王国栋..线粒体ATP合成酶复合体分离纯化的方法学研究与探讨[J].生命科学仪器,2007,5(1):25-31,7.基金项目
西北农林科技大学人才基金(2006) （2006）
