中国农业科学（英文版）2007，Vol.6Issue(1)：25-32,8.
Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum
Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum
摘要
Abstract
Three coding sequences of gliadins genes, designed as Gli2_Du1, Gli2_Du2 and Gli2_Du3, were isolated from the genomic DNA of Triticum durum accessions CItr5083. Gli2_Du1 and Gli2_Du2 contain 945 and 864 bp, encoding the mature proteins with 314 and 287 amino acid residues, respectively. Gli2_Du3 is recognized as a pseudogene due to the stop codon occurring in the coding region. The pseudogenes, commonly occurring in gliadins family, are attributed to the single base change C → T. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-gliadin proteins, including the toxic sequences (PSQQQP). The peptide fraction PF(Y)PP(Q)is thought to be an extra unit of repetitive domain, slightly diverging from the previous report. Six cysteine residues were observed within two unique domains. Phylogenetic analysis showed Gli2_Du2 and Gli2_Du3 were closely related to the genes on chromosome 6A, whereas Gli2_Du1 seems to be more homologous with the genes on chromosome 6B.关键词
durum wheat/α-gliadin/gene clone/sequence analysisKey words
durum wheat/α-gliadin/gene clone/sequence analysis分类
农业科技引用本文复制引用
WANG Han-yan,WEI Yu-ming ,ZE Hong-yan ,ZHENG You-liang..Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum[J].中国农业科学（英文版）,2007,6(1):25-32,8.基金项目
This work was supported by the National High Technology Research and Development Program of China (863 Program, 2006AA10Z179, 2006AA10ZEF87), National Natural Science Foundation of China (30300219 and 30571163),and the Foundation for the Authors of National Excellent Doctoral Dissertation of China (200357 and 200458) （863 Program, 2006AA10Z179, 2006AA10ZEF87）
