中国临床康复2004，Vol.8Issue(13)：2550-2551,2.
脐血单个核细胞培养前后神经细胞特有标志物的变化
Change of specific markers before and after culture of cord blood mononuclear cells
摘要
Abstract
BACKGROUND: The source of neural stem cells(NSCs) now just limits tothe embryos so that it is extremely important to seek a new resource of NSCSfor further developing both foundational and applied research in the field ofneuroscience. Cord blood is found a good source of NSCs.OBJECTIVE: To explore the expression difference on specific markers ofcord blood mononuclear cells(MNCs) before and after culture.SETTING and MATERIALS: The research was done in the Stem CellResearch Center in the Medical College of Zhengzhou University. 50 - 100mL cord blood sample was obtained aseptically from infants delivered by fullterm normal labor or cesarean in Obstetrical Department of First and ThirdAffiliated Hospitals by using blood collection bags containing anticoagulantheparin. After fully mixed, the blood sample was transported to laboratory in athermal insulation box.DESIGN and METHODS: Expressions of Nestin, NF-M, MAP2 and mRNAwere assayed by RT-PCR method before and after culture of cord bloodMNCs.MAIN OUTCOME MEASURES: Expressions of Nestin, NF-M, MAP2 andmRNA before and after the culture of cord blood MNCs.RESULTS: There were positive expressions of Nestin, NF-M, MAP2 andmRNA both before and after the culture of cord blood mononuclear cells.Compared with non-cultured MNCs, the expressions of Nestin, NF-M, MAP2and mRNA were enhanced in the cultured sample.CONCLUSION: There were neural stem cells existing in the directly iso-lated cord blood mononuclear cells. Afrer culture, the expressions of Nestin,NF-M, MAP2 and mRNA were enhanced because of the increasing number ofneuron-like cells.关键词
脐血/单个核细胞/干细胞/微管相关蛋白分类
医药卫生引用本文复制引用
邢莹,鄢文海,刘计荣,龚光明,许燕,张莹,曹孟德..脐血单个核细胞培养前后神经细胞特有标志物的变化[J].中国临床康复,2004,8(13):2550-2551,2.基金项目
河南省重点科技攻关基金资助项目01170319 （）
