南方医科大学学报2006，Vol.26Issue(3)：255-257,3.
5-氨基乙酰丙酸介导的光动力学治疗胃癌的实验研究
Effect of photodynamic therapy with 5-aminolevulinic acid on human gastric cancer cells in vitro
摘要
Abstract
Objective To investigate the effect of 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy (PDT) on MGC-803 human gastric cancer cells in vitro. Methods MGC-803 human gastric cancer cells were treated with 5-ALA at various concentrations followed by laser irradiation. The cells were also treated with 5-ALA at the same concentration before laser exposure at various doses. PDT-induced phototoxicity of the cells was determined by MTT assay. Results After laser exposure of the cells at the same dose (25.0 J/cm2), the cell survival rates decreased significantly with incubation of the cells with 5-ALA at 0.25, 0.5, 1.0, 2.0 and 4.0 mmol/L, respectively (F=266.39, P＜0.001), but 2.0 and 4.0 mmol/L ALA showed no significant difference in lowering the cell survival rates (P＞0.05). Following treatment with the same 5-ALA concentration (1 mmol/L), the cell survival rates decreased in response to increased laser doses (at 6.25, 12.5, 25.0, 50.0, and 100 J/cm2, respectively, F=226.31, P＜0.0001). Without laser exposure, the survival rate of the cells did not significantly change for different 5-ALA concentrations (F=0.79, P=0.5383), nor did it undergo obvious variation in response to different laser doses without 5-ALA incubation (F=0.61, P=0.6551). Conclusions The damage of MGC-803 cells by PDT increases with 5-ALA concentration within a relative lower range and is proportional to the laser doses delivered. Without 5-ALA treatment, the laser at the chosen dose cannot produce photodynamic effect and ALA itself is nontoxic. ALA-mediated PDT appears to be a promising therapy for gastric cancer.关键词
胃癌/5-氨基乙酰丙酸/5-ALA/光动力学治疗/MGC-803细胞Key words
gastric cancer/5-aminolevulinic acid/photodynamic therapy/MGC-803 cells分类
医药卫生引用本文复制引用
黄宗海,周广军,俞金龙,厉周,丁涟沭,徐如祥,姜晓丹..5-氨基乙酰丙酸介导的光动力学治疗胃癌的实验研究[J].南方医科大学学报,2006,26(3):255-257,3.基金项目
Supported by National "863"Development Project in High-Tech Research (2001AA217171) and Key Research Project of Natural Science Foundation of Guangdong Province (013072) （2001AA217171）
