中国肿瘤生物治疗杂志2000，Vol.7Issue(4)：255-260,6.
IL-6受体在人白血病细胞膜上的表达及亲和力
Density and Affinity of IL-6 Receptors in Human Leukemic Cells
摘要
Abstract
Objective: To make a study of density and affinity of IL-6R in human leukemic cell lines, and discuss the affection of high affinity IL-6R to the targeted treatment of leukemia with IL-6-PE40 fusion protein. Methods: Radial binding assay with scatchard plot and FACS were used to analysis the density and affinity of IL-6R and protein expression of IL-6Rα and β subunits in totally 8 representative human leukemic cell lines. Results: Myelocytie, monocytic and erythrocytic leukemic cell lines U937, HL-60, KG1 and TF1 express high affinity IL-6R, whose average density per cell is 2 502,2 874, 2 319 and 9 329 respectively, however no 125I-IL-6 binding was detected on chronic myelocytic leukemic cell line K562 and lymphoblastic leukemic cell lines such as Raji, CEM and HUT28. These results correlate with those of FACS highly. Conclusion:These observations suggest that acute nonlymphoblastic leukemic cells may be more suitable for targeted treatment with IL-6-PFA0 fusion protein.关键词
白血病细胞/IL-6受体/放射配体结合实验/亲和力Key words
leukemic cells/interleukin-6 receptor/radial ligand binding assay/affinity分类
医药卫生引用本文复制引用
刘爽,奚永志,郭斯启,刘楠,屠敏,金荔,陈兴国,孔繁华..IL-6受体在人白血病细胞膜上的表达及亲和力[J].中国肿瘤生物治疗杂志,2000,7(4):255-260,6.基金项目
本课题受国家自然科学基金(39500062)，(39870875)资助 （39500062）
