中国临床康复2004，Vol.8Issue(13)：2561-2563,3.
大鼠全脑缺血再灌注时左旋四氢巴马汀对脑内离子稳态平衡的影响
Influence of L-tetrahydropalmatine on rat brain ion homeostasis in the course of cerebral ischemia reperfusion
摘要
Abstract
BACKGROUND: Cerebral ischemia-reperfusion leads to disruption of brainion homeostasis that aggravates ischemia-reperfusion injury.OBJECTIVE: To investigate the action mechanism of L-tetrahydropalmatine(L-THP) in cerebral ischemia-reperfusion injury by observing the changes inthe contents of calcium, magnesium, zinc, sodium, potassium and iron in thebrain tissues.DESIGN: Completely randomized controlled study.SETTING and MATERIALS: This study was carried out in the EmergencyDepartment, Tongji Hospital, Huazhong University of Science and Technolo-gy. Thirty-five Wistar rats of clean grade weighing 180 - 200 g and aged 4 -6 weeks old were used.METHODS: Rat models of cerebral ischemia-reperfusion injury were estab-lished to determine the electrolyte contents using an atomic absorptionspectrophotometer ( AAS ).RESULTS: Compared with the sham operation group, the cerebral ischemia-reperfusion group, at 12 hours, had elevated levels of brain calcium[ (218.66 ±11.88) μg/g, q=5.526, P ＜0.01], zinc[(72.45±0.83) μg/g, q=23. 240, P ＜ 0.01 }, sodium[ (5 237.85 ± 106.48) μg/g, q = 7. 956, P ＜0.01], potassium[(15 421.52 ±264. 86) μg/g, q =3. 805, P ＜0.05] andiron[ (151.27 ± 10. 21 ) μg/g, q = 3. 392, P ＜ 0.05], but with lowered mag-nesium content; at 24 hours after the injury, further increases occurred in thelevels of calcium[(295. 63 ±64. 69) μg/g, q=12.251, P ＜0.01], zinc[(74.44±0.47) μg/g, q=27.354, P ＜0.01], sodium[ (5 056.68±100.18) μg/g, q=5.269, P ＜ 0.01] and potassium[(15 116.52±631.96) μg/g, q =4. 156, P ＜ 0. 05], while the content of magnesium[(587.14±10.90) μg/g, q=10.499, P ＜0.01] decreased, and thecontent of iron[(118. 79±14.22) μg/g, q=2.515, P＞ 0.05] wassimilar to that in the sham operation group. At both 12 and 24 hours after thereperfusion, L-THP inhibited the increase in the contents of calcium[ (175.67 ± 2.70) μg/g, q = 3. 756, P ＜ 0. 05; (190. 76 ± 4.60) μg/g,q=9.163, P ＜0.01], zinc[(66.77±1. 14) μg/g, q=11.758, P ＜0.01; (69.94 ± 0.98) μg/g, q = 9. 304, P ＜0.01] and sodium[ (4 841.94 ±179.00) μg/g, q=4.206, P ＜0.05; (4251.05±-194.31) μg/g, q=3. 183, P ＞ 0. 05], increased the contents of magnesium [ (706.21 ±-25.92) μg/g, q=15.888, P ＜ 0.01; (662.80±9.74) μg/g, q=13.585, P ＜0.01] and potassium[(16 294.68±102.48) μg/g, q=5. 274, P ＜ 0.01; (16 577.51 ± 152.46) μg/g, q = 3. 339, P ＞ 0.05], anddecreased the contents of iron[(86.90±2.89) μg/g, q=11.607, P ＜0.01;(84.85±3.21) μg/g, q=11.795, P ＜0.01].CONCLUSION: L-THP can inhibit the increase of calcium, zinc and sodiumcontents, increase the contents of magnesium and potassium, and decrease thecontent of iron in rat brain tissues during cerebral ischemia-reperfusion.`关键词
脑缺血再灌注/电解质/脑分类
医药卫生引用本文复制引用
杨光田,陈齐国..大鼠全脑缺血再灌注时左旋四氢巴马汀对脑内离子稳态平衡的影响[J].中国临床康复,2004,8(13):2561-2563,3.基金项目
湖北省卫生厅基金资助项目(WJ01518) （WJ01518）
