中国现代医学杂志2007，Vol.17Issue(21)：2561-2564,4.
参附注射液对兔心肌缺血再灌注损伤中内皮功能的影响
Effect of Shenfu injection on endothelium function during myocardial ischemia and reperfusion injury in rabbits
崔巍 1郭莲怡1
作者信息
- 1. 中国医科大学附属第一医院传染科,辽宁,沈阳,110001
- 折叠
摘要
Abstract
[Objective] To study the change of endothelial function during myocardial ischemia and reperfusion (I/R) injury of rabbits and the effect of Shenfu Injection (SFI) on it and the action mechanism. [Methods] 21 Japanese white rabbits were randomly divided into 3 groups (n =7 in each): group A, sham opreration; group B, I/R;group C, I/R treated with SFI. The concentration of serum nitric oxide product (NOP) and plasma endothelin(ET) before ligation of the coronary artery, at 40 minutes of ischemia and 40 minutes of reperfusion was determined. The concentration of total superoxide dismutase (T-SOD) and malonyl-dialdehyde(MDA) of myocardial tissue was determined. Ultra-microstructure of myocardial cells was observed with electro microscope. [Results] Serum NOP of group B decreased and plasma ET of it increased markedly compared with those of group A at 40 minutes of ischemia (P ＜0.01), more significantly at 40 minutes of reperfusion. NOP correlated positively with ET at 40 minutes of ischemia (P ＜0.05) and 40 minutes of reperfusion(P ＜0.01). At 40 minutes of reperfusion, T-SOD of group B was lower significantly than group A, MDA was significantly higher (P ＜0.01). NOP correlated positively with T-SOD and correlated negatively with MDA. ET correlated positively with MDA and correlated negatively with T-SOD. Ultramicrostructure of myocardial cells was abnormal. SFI can increase NOP at 40 minutes of ischemia and 40 minutes of reperfusion and lower ET at 40 minutes after reperfusion significantly (P ＜0.01). SFI can increase T-SOD and lower MDA significantly (P ＜0.01), the abnormality of myocardial cells can be reduced obviously. [Conclusion] SFI can improve the abnormal endothelial function in which free radicals are involved and reduce myocardial I/R injury.关键词
心肌缺血-再灌注损伤/一氧化氮/内皮缩血管肽/参附注射液Key words
myocradial ischemia/ reperfusion injury/ nitric oxide/ endothelin/ Shenfu injection分类
医药卫生引用本文复制引用
崔巍,郭莲怡..参附注射液对兔心肌缺血再灌注损伤中内皮功能的影响[J].中国现代医学杂志,2007,17(21):2561-2564,4.
