首页|期刊导航|中华医学杂志（英文版）|Effects of activated protein C on coagulation and fibrinolysis in rabbits with endotoxin induced acute lung injury
中华医学杂志（英文版）2008，Vol.121Issue(24)：2561-2565,5.
Effects of activated protein C on coagulation and fibrinolysis in rabbits with endotoxin induced acute lung injury
Effects of activated protein C on coagulation and fibrinolysis in rabbits with endotoxin induced acute lung injury
摘要
Abstract
Background Sepsis induced acute lung injury (ALl) as a common syndrome in clinical practice has a high mortality. Recombinant human activated protein C (APC) can significantly reduce the mortality of patients with severe sepsis. Several studies have implicated that APC may be protective in ALl. Methods Twenty-one rabbits were operatively prepared and randomly divided into sham, control, or APC groups (n=7 in each group). After a tracheotomy had been performed, ALl was produced in the control and APC groups by infusion of Escherichia coli endotoxin 100 pg/kg per hour intravenously for 1 hour. The sham group received only the vehicle, infusion of 20 ml of 0.9% saline. The rabbits were studied under anesthesia for 6 hours and were ventilated with 40% and lasted for 4 hours. The animals were resuscitated with Ringer's lactate solution. Results In comparison with nontreatment in the control group, the infusion of APC significantly reduced the increase of thrombomodulin level (TM; control group was (0.68±0.06) ng/ml, vs APC group of (0.62±0.07) ng/ml at 6 hours, P＜0.05), and significantly attenuated the fall in protein S (PS; control group was (2.32±0.03) μg/ml at 2 hours, (2.24±0.06) μg/ml at 4 hours and (2.21±0.09) μg/ml at 6 hours, vs APC group (2.46±0.04) μg/ml at 2 hours, (2.40±0.05) pg/ml at 4 hours and (2.39±0.07) μg/ml at 6 hours, P＜0.01). In addition, APC limited the increase in plasminogen activator inhibitor-1 (PAl-1) both in plasma (control group was (0.68±0.12) ng/ml at 1 hour, (0.84±0.06) ng/ml at 2 hours, (0.87±0.08) ng/ml at 4 hours and (0.91±0.05) ng/ml at 6 hours, vs APC group (0.42±0.16) ng/ml at 1 hour, (0.43±0.04) ng/ml at 2 hours, (0.45±0.09) ng/ml at 4 hours and (0.45±0.14) ng/ml at 6 hours, P＜0.01) and in bronchoalveolar lavage fluid (at 6 hours: sham, (1.05±0.05) ng/ml; control, (1.13±0.06) ng/ml; APC, (1.06±0.06) ng/ml; P＜0.05). However, APC failed to prevent the decrease in PaO<,2>/FiO<,2> ratio. APC-treated rabbits showed no significant difference in platelet count and antithrombin but exhibited less D-dimer production than did the controls. Moreover, APC limited the histopathological score of lung injury (2.6±0.8 in control, vs 1.4±0.6 in APC group, P＜0.01). Conclusion Anti-coagulation and pro-fibrinolysis activity may be two of the possible mechanisms by which activated protein C attenuated endotoxin-induced ALl.关键词
acute lung injury/sepsis/activated protein C/coagulation/fibrinolysisKey words
acute lung injury/sepsis/activated protein C/coagulation/fibrinolysis分类
医药卫生引用本文复制引用
..Effects of activated protein C on coagulation and fibrinolysis in rabbits with endotoxin induced acute lung injury[J].中华医学杂志（英文版）,2008,121(24):2561-2565,5.基金项目
This project was supported by the Beijing Natural Science Foundation, China (No. 7032019). （No. 7032019）
