中国免疫学杂志2001，Vol.17Issue(5)：256-258,3.
血小板因子4基因转染对肺癌细胞中血管生成因子基因表达的影响
Effects of transduction hPF4 gene on the angiogenesis factors gene expression in human lung cancer cells
郝好杰 1谷志远 1刘文英 1宋海静 1赵亚力 1李琦1
作者信息
- 1. 解放军总医院基础医学研究所分子生物学研究室,
- 折叠
摘要
Abstract
Objective: To study the inhibiting angiogenesis mechanism of human platelet factor 4(hPF4).Methods:A hPF4 gene recombinant expression vector was constructed by cloning hPF4 cDNA into the pcDNA3 vector, and was transferred by lipofectamin into hPF4 deficient human pulmonary giant cell carcinoma ecll line (PLA801D) with lipofectamin. The mRNA and protein expression level of PLA801D-hPF4 cell was detected by RT-PCR and immunocytochemistry. Results:Stable expression of hPF4 mRNA in PLA801D-hPF4 cells was confirmed by RT PCR. The angiogenic factor VEGF、bFGF、IL-8 mRNA and protein expression of PLA801D-hPF4 cell was significantly inhibited compared with the controls. These results demonstrate that transfection of hPF4 gene into lung cancer cells could reduce its angiogenic factors mRNA expression, so it also effects the compositinn of their protein. Conclusion:These date indicated that down-regulated mRNA expression of the angiogenic factor in cancer eclls can be one of the mecharnisms of inhibiting angiogenesis of hPF4.关键词
人血小板因子4肿瘤血管生成因子分类
医药卫生引用本文复制引用
郝好杰,谷志远,刘文英,宋海静,赵亚力,李琦..血小板因子4基因转染对肺癌细胞中血管生成因子基因表达的影响[J].中国免疫学杂志,2001,17(5):256-258,3.
