棉花学报2008,Vol.20Issue(4):256-263,8.
棉属野生种克劳茨基棉(Gossypium klotzschianum)基因组向陆地棉的渐渗
Introgression of Gossypium klotzschianum Genome into Cultivated Cotton, G. hirsutum
摘要
Abstract
Exotic Gossypium germplasms are rich sources of useful agronomic traits for improvement programs of cultivated cotton. Efficient use of genetic variation available in the wild relatives depends on the ability to identify and introgress desirable DNA segments from wild species into cultivated cotton. To introgress genes from G. klotzschianum into G. hirsutum, a BC1 F2 population from (G. hirsutum×G. klotzschianum) ×G. hirsutum was constructed. A total of 320 SSR primers covering the cotton genome were selected to monitor alien germplasm transmission. Only 38 markers showed segregation in the BC1 F2 population, and they were distributed in 14 different chromosomes and constituted 18 introgressed segments. The total length of the 18 introgressed segments was 595 cM based on a cotton consensus map. Two morphological traits (yellow petal and open bud)were mapped on chromosome 13. Our molecular and morphological identification confirmed the presence of G. klotzschianum chromatin in the BC1 F2 interspecific progenies. The use of species-specific molecular markers will greatly facilitate introgression of desirable alien genes into cultivated cotton.关键词
克劳茨基棉/SSR/渐渗/分子标记Key words
G. klotzschianum/SSR/introgressed segment/species-specific molecular markers分类
农业科技引用本文复制引用
沈新莲,朱静,张香桂,张保龙,曹志斌,杨郁文,徐鹏,徐英俊,倪万潮..棉属野生种克劳茨基棉(Gossypium klotzschianum)基因组向陆地棉的渐渗[J].棉花学报,2008,20(4):256-263,8.基金项目
江苏省自然科学基金(BK2005433) (BK2005433)