中国临床康复2004，Vol.8Issue(13)：2564-2565,2.
甲壳胺膜管修复不同长度神经缺损的实验研究
An experimental study of repairing nerve defect indifferent lengths with chitosan membranous tube
胡勇 1刘玉玲 2董吟林 1高学纯 1卜海富 1尹宗生1
作者信息
- 1. 安徽医科大学,第一附属医院骨科,安徽省合肥市,230022
- 2. 安徽医科大学, 外科总论实验室,安徽省合肥市,230022
- 折叠
摘要
Abstract
BACKGROUND: Recently, the reports about the application of naturaldegradable biomaterial in peripheral nerve surgery field, especially the rela-tionship between the severity of nerve injury and the indication of biologicalductus, were rare.OBJECTIVE: To observe the impact of bridging the sciatic nerve defect indifferent lengths with chitosan membranous tube on nerve regeneration in therat and to explore the indication of this method in the repair of nerve injurywith different severity.DESIGN: A completely randomized controlled study.SETTING and MATERIALS: The study was completed in the Experi-mental Surgery Center of Anhui Medical University. Subjects were SD rats(grade cleanness) in either gender with a body weight from 220 g to 240 gobtained from Experimental Animal Center of Anhui Medical University.INTERVENTIONS: Totally 102 SD rats were allocated into four-time defect(4-fold defect), six-time defect(6-fold defect) and eight-time defect(8-folddefect) groups according to the ratio of the length of defect to the diameter ofnerve stem. Inter-control was introduced. Defects were bridged by chitosanmembranous tube. Animals were fed in laminar flow room and executed at 4,8 and 12 weeks.MAIN OUTCOME MEASURES: General observations, ultrastructure ob-servations under transmission electron microscope, histology observations andthe comparison in the regeneration rates of axons at distal end were assayedrespectively at 4, 8 and 12 weeks after the surgery.RESULTS: 4-fold and 6-fold defect groups had toe spread-out and musclecontraction. There was no scar adhesion around bridging. The result of4-fold-defect group was better than that of 6-fold defect and 8-fold defectgroups. 6-fold defect group was better than that of 8-fold defect group. Thematurities of myelin sheath in 4-fold and 6-fold defect groups were good, whilethere was still ulceration in myelin sheath in 8-fold defect group. There were nocollagen hyperplasias in all 3 groups.CONCLUSION: Peripheral nerve defect bridged by chitosan membranoustube could prevent the invasion of scar tissue, which is helpful to the regen-eration of axons. The quality and quantity of regenerated axons after thebridging by chitosan membranous tube were preferably good if the length ofnerve defect did not exceed 6 times as its diameter.关键词
壳多糖/周围神经/创伤和损伤/修复外科手术分类
医药卫生引用本文复制引用
胡勇,刘玉玲,董吟林,高学纯,卜海富,尹宗生..甲壳胺膜管修复不同长度神经缺损的实验研究[J].中国临床康复,2004,8(13):2564-2565,2.
